Genome-Wide Identification of Reference Genes for Reverse-Transcription Quantitative PCR in Goat Rumen.

ABSTRACT: As the largest chamber of the ruminant stomach, the rumen not only serves as the principal absorptive surface and nutrient transport pathway from the lumen into the animal, but also plays an important short-chain fatty acid (SCFA) metabolic role in addition to protective functions. Accurate characterization of the gene expression profiles of genes of interest is essential to the exploration of the intrinsic regulatory mechanisms of rumen development in goats. Thus, the selection of suitable reference genes (RGs) is an important prerequisite for real-time quantitative PCR (RT-qPCR). In the present study, 16 candidate RGs were identified from our previous transcriptome sequencing of caprine rumen tissues. The quantitative expressions of the candidate RGs were measured using the RT-qPCR metho

SUBMITTER: Zhao J 

PROVIDER: S-EPMC8614340 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature