Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid.

ABSTRACT: Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three signific

SUBMITTER: Ding Q 

PROVIDER: S-EPMC8614480 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature