Oxford Nanopore MinION Direct RNA-Seq for Systems Biology.

ABSTRACT: Long-read direct RNA sequencing developed by Oxford Nanopore Technologies (ONT) is quickly gaining popularity for transcriptome studies, while fast turnaround time and low cost make it an attractive instrument for clinical applications. There is a growing interest to utilize transcriptome data to unravel activated biological processes responsible for disease progression and response to therapies. This trend is of particular interest for precision medicine which aims at single-patient analysis. Here we evaluated whether gene abundances measured by MinION direct RNA sequencing are suited to produce robust estimates of pathway activation for single sample scoring methods. We performed multiple RNA-seq analyses for a single sample that originated from the HepG2 cell line, namely five ONT repli

SUBMITTER: Pyatnitskiy MA 

PROVIDER: S-EPMC8615092 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature