Estimating Drug Efficacy with a Diet-Induced NASH Model in Chimeric Mice with Humanized Livers.

ABSTRACT: Nonalcoholic fatty liver disease/steatohepatitis (NAFLD/NASH) is the most common liver disorder in developed countries. Although many new therapeutics for NASH are present in the drug development pipeline, there are still no approved drugs. One of the reasons that makes NASH drug development challenging is the lack of appropriate animal NASH models that resolve issues arising from inter-species differences between humans and rodents. In the present study, we developed a choline-deficient, L-amino-acid-defined, high-fat-diet (CDAHFD)-induced human NASH model using human liver chimeric mice. We demonstrated human hepatocyte injury by an elevation of plasma human alanine aminotransferase 1 in mice fed CDAHFD. Histological analysis showed that CDAHFD feeding induced similar histological change

SUBMITTER: Kisoh K 

PROVIDER: S-EPMC8615377 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature