Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM).

ABSTRACT: High resolution episcopic microscopy (HREM) produces digital volume data by physically sectioning histologically processed specimens, while capturing images of the subsequently exposed block faces. Our study aims to systematically define the spectrum of typical artefacts inherent to HREM data and to research their effect on the interpretation of the phenotype of wildtype and mutant mouse embryos. A total of 607 (198 wildtypes, 409 mutants) HREM data sets of mouse embryos harvested at embryonic day (E) 14.5 were systematically and comprehensively examined. The specimens had been processed according to essentially identical protocols. Each data set comprised 2000 to 4000 single digital images. Voxel dimensions were 3 × 3 × 3 µm³. Using 3D volume models and virtual resections, we i

SUBMITTER: Reissig LF 

PROVIDER: S-EPMC8615656 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature