Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.

ABSTRACT: Understanding seizure development requires an integrated knowledge of different scales of organization of epileptic networks. We developed a model of "epilepsy-in-a-dish" based on dissociated primary neuronal cells from neonatal rat hippocampus. We demonstrate how a single application of glutamate stimulated neurons to generate spontaneous synchronous spiking activity with further progression into spontaneous seizure-like events after a distinct latency period. By computational analysis, we compared the observed neuronal activity in vitro with intracranial electroencephalography (EEG) data recorded from epilepsy patients and identified strong similarities, including a related sequence of events with defined onset, progression, and termination. Next, a link between the neurophysiological ch

SUBMITTER: Jablonski J 

PROVIDER: S-EPMC8616120 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature