Temporal and Site-Specific ADP-Ribosylation Dynamics upon Different Genotoxic Stresses.

ABSTRACT: The DNA damage response revolves around transmission of information via post-translational modifications, including reversible protein ADP-ribosylation. Here, we applied a mass-spectrometry-based Af1521 enrichment technology for the identification and quantification of ADP-ribosylation sites as a function of various DNA damage stimuli and time. In total, we detected 1681 ADP-ribosylation sites residing on 716 proteins in U2OS cells and determined their temporal dynamics after exposure to the genotoxins H2O2 and MMS. Intriguingly, we observed a widespread but low-abundance serine ADP-ribosylation response at the earliest time point, with later time points centered on increased modification of the same sites. This suggests that early serine ADP-ribosylation events may serve as a platform for

SUBMITTER: Buch-Larsen SC 

PROVIDER: S-EPMC8616546 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature