Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans.

ABSTRACT: The germ line produces gametes that transmit genetic and epigenetic information to the next generation. Maintenance of germ cells and development of gametes require germ granules-well-conserved membraneless and RNA-rich organelles. The composition of germ granules is elusive owing to their dynamic nature and their exclusive expression in the germ line. Using Caenorhabditis elegans germ granule, called P granule, as a model system, we employed a proximity-based labeling method in combination with mass spectrometry to comprehensively define its protein components. This set of experiments identified over 200 proteins, many of which contain intrinsically disordered regions (IDRs). An RNA interference-based screen identified factors that are essential for P granule assembly, notably EGGD

SUBMITTER: Price IF 

PROVIDER: S-EPMC8616582 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature