Single-cell analyses of human pancreas: characteristics of two populations of acinar cells in chronic pancreatitis.

ABSTRACT: Chronic pancreatitis (CP) is a complex inflammatory disorder with numerous associated genetic and environmental risk factors. The most distressing characteristic of CP is recalcitrant pain, often requiring surgical resection including total pancreatectomy with islet autotransplantation (TPIAT). We studied five consented subjects undergoing pancreatic resection and processed isolated cells for single-cell RNA sequencing (scRNA-Seq). Using high-dimensional transcriptomic cluster analysis, we identified 11 unique cell clusters in the pancreas tissue. These cell clusters include a cluster of undifferentiated/dedifferentiated cells and two unique clusters of acinar cells, one of which appears to be in a transitional stage. To determine the cellular response to protease inhibitor and stimulation

SUBMITTER: Blobner BM 

PROVIDER: S-EPMC8616588 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature