Hepatic insulin-degrading enzyme regulates glucose and insulin homeostasis in diet-induced obese mice.

ABSTRACT: The insulin-degrading enzyme (IDE) is a metalloendopeptidase with a high affinity for insulin. Human genetic polymorphisms in Ide have been linked to increased risk for T2DM. In mice, hepatic Ide ablation causes glucose intolerance and insulin resistance when mice are fed a regular diet.

Objective

These studies were undertaken to further investigate its regulatory role in glucose homeostasis and insulin sensitivity in diet-induced obesity.

Methods

To this end, we have compared the metabolic effects of loss versus gain of IDE function in mice fed a high-fat diet (HFD).

Results

We demonstrate that loss of IDE function in liver (L-IDE-KO mouse) exacerbates hyperinsulinemia and insulin resistance without changes in insulin clearance but in parallel to an increase in pancre

SUBMITTER: Merino B 

PROVIDER: S-EPMC8616598 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature