Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary western blot technology and total protein normalization.

ABSTRACT: The western blot (WB) is the predominate method for protein quantification, frequently used in pharmacological and toxicological studies. To control for technical variation, WB signals are normalized through immunodetection of an internal standard "house-keeping" gene or total protein quantification via staining of the same blot or a duplicate, sister blot. Increasing evidence suggests that house-keeping genes are subject to change after drug treatment or under disease states, causing protein quantification errors in WB. Recent advances in automated capillary-based WB technologies enable measurement of the protein of interest, internal standards, and total protein in a single capillary. Using this approach, we quantified cytochrome P450 3A4 (CYP3A4) across 179 liver samples and compared no

SUBMITTER: Collins JM 

PROVIDER: S-EPMC8616831 | biostudies-literature | 2021 Nov-Dec

REPOSITORIES: biostudies-literature