Room-temperature dynamic nuclear polarization enhanced NMR spectroscopy of small biological molecules in water.

ABSTRACT: Nuclear magnetic resonance (NMR) spectroscopy is a powerful and popular technique for probing the molecular structures, dynamics and chemical properties. However the conventional NMR spectroscopy is bottlenecked by its low sensitivity. Dynamic nuclear polarization (DNP) boosts NMR sensitivity by orders of magnitude and resolves this limitation. In liquid-state this revolutionizing technique has been restricted to a few specific non-biological model molecules in organic solvents. Here we show that the carbon polarization in small biological molecules, including carbohydrates and amino acids, can be enhanced sizably by in situ Overhauser DNP (ODNP) in water at room temperature and at high magnetic field. An observed connection between ODNP ¹³C enhancement factor and paramagnetic <

SUBMITTER: Dai D 

PROVIDER: S-EPMC8616939 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature