Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis.

ABSTRACT: Membrane proteins are essential for cellular growth, signalling and homeostasis, making up a large proportion of therapeutic targets. However, the necessity for a solubilising agent to extract them from the membrane creates challenges in their structural and functional study. Although amphipols have been very effective for single-particle electron cryo-microscopy (cryoEM) and mass spectrometry, they rely on initial detergent extraction before exchange into the amphipol environment. Therefore, circumventing this pre-requirement would be a big advantage. Here we use an alternative type of amphipol: a cycloalkane-modified amphiphile polymer (CyclAPol) to extract Escherichia coli AcrB directly from the membrane and demonstrate that the protein can be isolated in a one-step purification with th

SUBMITTER: Higgins AJ 

PROVIDER: S-EPMC8617058 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature