Two novel human anti-CD25 antibodies with antitumor activity inversely related to their affinity and in vitro activity.

ABSTRACT: High tumor regulatory T (Treg) cell infiltration is associated with poor prognosis of many cancers. CD25 is highly expressed on tumor Treg cells and is a potential target for Treg deletion. Previously characterized anti-CD25 antibodies appear to have limited efficacy in tumor inhibition. Here we identified two human anti-CD25 antibodies, BA9 and BT942, which did not prevent the activation of IL-2R signaling pathway by IL-2. BT942 had weaker binding and cytotoxic activity to human CD25-expressing cell lines than BA9. But both demonstrated significant tumor growth inhibition in early and late-stage animal cancer models. BT942 resulted in a higher expansion of CD8⁺ T cells and CD4⁺ T cells in tumor microenvironment in mouse MC38 model compared to BA9. BT942 also demonstr

SUBMITTER: Song D 

PROVIDER: S-EPMC8617198 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature