Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling.

ABSTRACT: Soil microorganisms determine the fate of soil organic matter (SOM), and their activities compose a major component of the global carbon (C) cycle. We employed a multisubstrate, DNA-stable isotope probing experiment to track bacterial assimilation of C derived from distinct sources that varied in bioavailability. This approach allowed us to measure microbial contributions to SOM processing by measuring the C assimilation dynamics of diverse microorganisms as they interacted within soil. We identified and tracked 1,286 bacterial taxa that assimilated ¹³C in an agricultural soil over a period of 48 d. Overall ¹³C-assimilation dynamics of bacterial taxa, defined by the source and timing of the ¹³C they assimilated, exhibited low phylogenetic conservation. We i

SUBMITTER: Barnett SE 

PROVIDER: S-EPMC8617410 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature