Next- and Third-Generation Sequencing Outperforms Culture-Based Methods in the Diagnosis of Ascitic Fluid Bacterial Infections of ICU Patients.

ABSTRACT:

Objectives

Infections of the ascitic fluid are serious conditions that require rapid diagnosis and treatment. Ascites is often accompanied by other critical pathologies such as gastrointestinal bleeding and bowel perforation, and infection increases the risk of mortality in intensive care patients. Owing to a relatively low success rate of conventional culture methods in identifying the responsible pathogens, new methods may be helpful to guide antimicrobial therapy and to refine empirical regimens. Here, we aim to assess outcomes and to identify responsible pathogens in ascitic fluid infections, in order to improve patients' care and to guide empirical therapy.

Methods

Between October 2019 and March 2021, we prospectively collected 50 ascitic fluid samples from ICU patients

SUBMITTER: Goelz H 

PROVIDER: S-EPMC8617993 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature