Functional Importance of Hydrophobic Patches on the Ebola Virus VP35 IFN-Inhibitory Domain.

ABSTRACT: Viral protein 35 (VP35) of Ebola virus (EBOV) is a multifunctional protein that mainly acts as a viral polymerase cofactor and an interferon antagonist. VP35 interacts with the viral nucleoprotein (NP) and double-stranded RNA for viral RNA transcription/replication and inhibition of type I interferon (IFN) production, respectively. The C-terminal portion of VP35, which is termed the IFN-inhibitory domain (IID), is important for both functions. To further identify critical regions in this domain, we analyzed the physical properties of the surface of VP35 IID, focusing on hydrophobic patches, which are expected to be functional sites that are involved in interactions with other molecules. Based on the known structural information of VP35 IID, three hydrophobic patches were identified on its

SUBMITTER: Kasajima N 

PROVIDER: S-EPMC8618116 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature