Characterization of Escherichia coli from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate.

ABSTRACT: Insects as novel foods are gaining popularity in Europe. Regulation (EU) 2015/2283 laid the framework for the application process to market food insects in member states, but potential hazards are still being evaluated. The aim of this study was to investigate samples of edible insect species for the presence of antimicrobial-resistant and Shiga toxin-producing Escherichia coli (STEC). Twenty-one E. coli isolates, recovered from samples of five different edible insect species, were subjected to antimicrobial susceptibility testing, PCR-based phylotyping, and macrorestriction analysis. The presence of genes associated with antimicrobial resistance or virulence, including stx1, stx2, and eae, was investigated by PCR. All isolates were subjected to genome se

SUBMITTER: Muller A 

PROVIDER: S-EPMC8618678 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature