Evaluation of Sample Preparation Strategies for Human Milk and Plasma Proteomics.

ABSTRACT: Sample preparation is the most critical step in proteomics as it directly affects the subset of proteins and peptides that can be reliably identified and quantified. Although a variety of efficient and reproducible sample preparation strategies have been developed, their applicability and efficacy depends much on the biological sample. Here, three approaches were evaluated for the human milk and plasma proteomes. Protein extracts were digested either in an ultrafiltration unit (filter-aided sample preparation, FASP) or in-solution (ISD). ISD samples were desalted by solid-phase extraction prior to nRPC-ESI-MS/MS. Additionally, milk and plasma samples were directly digested by FASP without prior protein precipitation. Each strategy provided inherent advantages and disadvantages for milk and

SUBMITTER: Milkovska-Stamenova S 

PROVIDER: S-EPMC8618985 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature