Intratumoral Heterogeneity Promotes Collective Cancer Invasion through NOTCH1 Variation.

ABSTRACT: Cellular and molecular heterogeneity within tumors has long been associated with the progression of cancer to an aggressive phenotype and a poor prognosis. However, how such intratumoral heterogeneity contributes to the invasiveness of cancer is largely unknown. Here, using a tumor bioengineering approach, we investigate the interaction between molecular subtypes within bladder microtumors and the corresponding effects on their invasiveness. Our results reveal heterogeneous microtumors formed by multiple molecular subtypes possess enhanced invasiveness compared to individual cells, even when both cells are not invasive individually. To examine the molecular mechanism of intratumoral heterogeneity mediated invasiveness, live single cell biosensing, RNA interference, and CRISPR-Cas9 gene edi

SUBMITTER: Torab P 

PROVIDER: S-EPMC8619970 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature