Transcriptomic Characterization Reveals Attributes of High Influenza Virus Productivity in MDCK Cells.

ABSTRACT: The Madin-Darby Canine Kidney (MDCK) cell line is among the most commonly used cell lines for the production of influenza virus vaccines. As cell culture-based manufacturing is poised to replace egg-based processes, increasing virus production is of paramount importance. To shed light on factors affecting virus productivity, we isolated a subline, H1, which had twice the influenza virus A (IAV) productivity of the parent (P) through cell cloning, and characterized H1 and P in detail on both physical and molecular levels. Transcriptome analysis revealed that within a few hours after IAV infection, viral mRNAs constituted over one fifth of total mRNA, with several viral genes more highly expressed in H1 than P. Functional analysis of the transcriptome dynamics showed that H1 and P responded

SUBMITTER: Ye Q 

PROVIDER: S-EPMC8620111 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature