Detection of Low Density Lipoprotein-Comparison of Electrochemical Immuno- and Aptasensor.

ABSTRACT: An elevated level of low density lipoprotein (LDL) can lead to the cardiovascular system-related diseases, such as atherosclerosis and others. Therefore, fast, simple, and accurate methods for LDL detection are very desirable. In this work, the parameters characterizing the electrochemical immuno-and aptasensor for detection of LDL have been compared for the first time. An immunosensor has been designed, for which the anti-apolipoprotein B-100 antibody was covalently attached to 4-aminothiophenol (4-ATP) on the surface of the gold electrode. In the case of an aptasensor, the gold electrode was modified in a mixture of ssDNA aptamer specific for LDL modified with -SH group and 6-mercaptohexanol. Square-wave voltammetry has been used for detection of LDL in PBS containing redox active marker

SUBMITTER: Rudewicz-Kowalczyk D 

PROVIDER: S-EPMC8620298 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature