Rapid method towards proteomic analysis of dried blood spots by MALDI mass spectrometry.

ABSTRACT: Neonatal dried blood spots (DBS) are routinely utilized in the clinical setting as a diagnostic tool for various genetic disorders and infectious diseases. DBS allow for minimally invasive, small volume blood collection and are stored at room temperature. Neonatal whole blood and serum samples can be important in determining genetic risk factors and predicting infantile disease; however, at the present time, limited methods exist for rapidly analyzing DBS samples for their proteomic profile, years after samples have been collected. A novel method is presented for the extraction and analysis of target proteins and peptides from neonatal DBS using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Extraction parameters were optimized to achieve ideal

SUBMITTER: Samenuk GM 

PROVIDER: S-EPMC8620524 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature