Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication.

ABSTRACT: Human-induced pluripotent stem cell-derived cardiomyocytes are a potentially unlimited cell source and promising patient-specific in vitro model of cardiac diseases. Yet, these cells are limited by immaturity and population heterogeneity. Current in vitro studies aiming at better understanding of the mechanical and chemical cues in the microenvironment that drive cellular maturation involve deformable materials and precise manipulation of the microenvironment with, for example, micropatterns. Such microenvironment manipulation most often involves microfabrication protocols which are time-consuming, require cleanroom facilities and photolithography expertise. Here, we present a method to increase the scale of the fabrication pipeline, thereby enabling large-batch generation of shelf-stable

SUBMITTER: Kim AA 

PROVIDER: S-EPMC8620583 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature