RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test.

ABSTRACT:

Background

Nucleic acid-based amplification tests (NAAT), above all (q)PCR, have been applied for the detection of Mycobacterium leprae in leprosy cases and household contacts with subclinical infection. However, their application in the field poses a range of technical challenges. Loop-mediated isothermal amplification (LAMP), as a promising point-of-care NAAT does not require sophisticated laboratory equipment, is easy to perform, and is applicable for decentralized diagnosis at the primary health care level. Among a range of gene targets, the M. leprae specific repetitive element RLEP is regarded as highly sensitive and specific for diagnostic applications.  METHODS: Our group developed and validated a dry-reagent-based (DRB) RLEP LAMP, provided product specifications for custom

SUBMITTER: Saar M 

PROVIDER: S-EPMC8620619 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature