Comparison of four clinically validated testosterone LC-MS/MS assays: Harmonization is an attainable goal.

ABSTRACT:

Introduction

Immunoassays and liquid chromatography-tandem mass spectrometry assays are commonly employed in clinical laboratories for measurement of total testosterone in serum. Results obtained from either of these methodologies compare poorly due to differences in calibration and/or inadvertent detection of interfering substances by the immunoassays. Standardization efforts are underway, but recent studies indicate that accuracy remains an issue.

Methods

This study compares the results from four independently developed and validated LC-MS/MS assays for total testosterone. The calibration for each assay was verified using National Institute of Standards and Technology Standard Reference Material 971.

Results

Initially, one of the four assays had a mean percent diffe

SUBMITTER: French D 

PROVIDER: S-EPMC8620848 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature