Development of a CDC-certified total testosterone assay for adult and pediatric samples using LC-MS/MS.

ABSTRACT:

Background

Highly accurate and sensitive method to measure testosterone in hypogonadal male, female and children is vital for proper diagnosis of hormone-related conditions and their treatment.

Objective

To develop an accurate and robust total testosterone ESI-LC-MS/MS quantification method with a simple sample preparation workflow and sufficient sensitivity for serum or plasma samples of all gender and age groups, via ketone functional group derivatization (using Amplifex™ Keto Reagent).

Method

A simple sample preparation method to accommodate both low and high numbers of samples was developed using simultaneous protein precipitation and derivatization with Amplifex™ Keto reagent, followed by centrifugation and direct injection of supernatant into an LC-MS/MS system

SUBMITTER: Star-Weinstock M 

PROVIDER: S-EPMC8620864 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature