The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens.

ABSTRACT: Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagnostics is a challenge and sometimes nearly impossible. With the advent of isothermal amplification methods, which provide amplification of nucleic acids at a certain temperature and do not require thermocyclic equipment, going beyond the laboratory has become a reality for molecular diagnostics. The amplification stage ceases to be limited by time and instruments. Challenges to solve involve finding suitable approaches for rapid and user-friendly plant preparation and detection of amplicons after

SUBMITTER: Ivanov AV 

PROVIDER: S-EPMC8621059 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature