Functional Characterisation of Bile Metagenome: Study of Metagenomic Dark Matter.

ABSTRACT: Gallbladder metagenome involves a wide range of unidentified sequences comprising the so-called metagenomic dark matter. Therefore, this study aimed to characterise three gallbladder metagenomes and a fosmid library with an emphasis on metagenomic dark matter fraction. For this purpose, a novel data analysis strategy based on the combination of remote homology and molecular modelling has been proposed. According to the results obtained, several protein functional domains were annotated in the metagenomic dark matter fraction including acetyltransferases, outer membrane transporter proteins, membrane assembly factors, DNA repair and recombination proteins and response regulator phosphatases. In addition, one deacetylase involved in mycothiol biosynthesis was found in the metagenomic dark ma

SUBMITTER: Sabater C 

PROVIDER: S-EPMC8621414 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature