Development of an Efficient Extraction Method for Harvesting Gymnodimine-A from Large-Scale Cultures of <i>Karenia selliformis</i>.

ABSTRACT: Gymnodimine-A (GYM-A) is a fast-acting microalgal toxin and its production of certified materials requires an efficient harvesting technology from the large-scale cultures of toxigenic microalgae. In this study the recoveries of GYM-A were compared between several liquid-liquid extraction (LLE) treatments including solvents, ratios and stirring times to optimize the LLE technique for harvesting GYM-A from Karenia selliformis cultures, of which the dichloromethane was selected as the extractant and added to microalgal cultures at the ratio 55 mL L^-1 (5.5%, v/v). The recovery of GYM-A obtained by the LLE technique was also compared with filtration and centrifugation methods. The stability of GYM-A in culture media were also tested under different pH conditions

SUBMITTER: Tang Z 

PROVIDER: S-EPMC8621799 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature