Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode.

ABSTRACT: Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment

SUBMITTER: Kulikova T 

PROVIDER: S-EPMC8621912 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature