Model Balancing: A Search for In-Vivo Kinetic Constants and Consistent Metabolic States.

ABSTRACT: Enzyme kinetic constants in vivo are largely unknown, which limits the construction of large metabolic models. Given measured metabolic fluxes, metabolite concentrations, and enzyme concentrations, these constants may be inferred by model fitting, but the estimation problems are hard to solve if models are large. Here we show how consistent kinetic constants, metabolite concentrations, and enzyme concentrations can be determined from data if metabolic fluxes are known. The estimation method, called model balancing, can handle models with a wide range of rate laws and accounts for thermodynamic constraints between fluxes, kinetic constants, and metabolite concentrations. It can be used to estimate in-vivo kinetic constants, to complete and adjust available data, and to construct plausible m

SUBMITTER: Liebermeister W 

PROVIDER: S-EPMC8621975 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature