Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium Hafnia alvei.

ABSTRACT: Quorum sensing (QS) is widely present in microorganisms in marine aquatic products. Owing to the use of antibiotics, many spoilage bacteria in aquatic products are drug resistant. In order to slow down this evolutionary trend, the inhibition of spoilage phenotype of spoilage bacteria by interfering with QS has become a research hot spot in recent years. In this study, we found a new QS quenching enzyme, PF-1240; it was cloned and expressed in Pseudomonas fluorescens 08. Sequence alignment showed that its similarity with N-homoserine lactone (AHL) acylase QuiP protein of Pseudomonas fluorescens (Pf 0-1) was 78.4%. SDS-PAGE confirmed that the protein is a dimer composed of two subunits, which is similar to the structure of AHL acylases. The concentration of heterologous expression in Escheri

SUBMITTER: Shen Y 

PROVIDER: S-EPMC8622684 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature