Theoretical and Experimental Investigation of Alginate Microtube Extrusion for Cell Culture Applications.

ABSTRACT: A novel cell culture technology, consisting of hollow alginate tubes, OD ~550 μm, ID ~450μm containing a cell suspension, provides stress-free conditions. Cells reach confluency in approximately ten days with cell densities of 0.5 - 1 billion cells per mL. Tubes are manufactured in a tri-axial needle extruder with three concentric flows. The cell suspension flows in the inner needle (N1), the alginate solution flows in the annulus between N1 and the second needle (N2) and a CaCl ₂ solution is the sheath fluid between the second and third needle (N3). Beyond the tip of N2, the sheath solution is in contact with the alginate and Ca ²⁺ diffuses into the alginate solution and crosslinks it to form an alginate microtube around the core fl

SUBMITTER: Nusterer M 

PROVIDER: S-EPMC8622871 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature