Nanoencapsulation as a General Solution for Lyophilization of Labile Substrates.

ABSTRACT: Protein macromolecules occur naturally at the nanoscale. The use of a dedicated nanoparticle as a lyophilization excipient, however, has not been reported. Because biopolymeric and lipid nanoparticles often denature protein macromolecules and commonly lack the structural rigidity to survive the freeze-drying process, we hypothesized that surrounding an individual protein substrate with a nanoscale, thermostable exoshell (tES) would prevent aggregation and protect the substrate from denaturation during freezing, sublimation, and storage. We systematically investigated the properties of tES, including secondary structure and its homogeneity, throughout the process of lyophilization and found that tES have a near 100% recovery following aqueous reconstitution. We then tested the hypothesis th

SUBMITTER: Vallerinteavide Mavelli G 

PROVIDER: S-EPMC8622885 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature