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2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology.

ABSTRACT: 2A is an oligopeptide sequence that mediates a ribosome "skipping" effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed.

SUBMITTER: de Lima JGS

PROVIDER: S-EPMC8623073 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology.

de Lima Juliana G S JGS Lanza Daniel C F DCF

Viruses 20211026 11

2A is an oligopeptide sequence that mediates a ribosome "skipping" effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different ...[more]

PMID: 34834965

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