Pyrophosphate-Enhanced Oxidase Activity of Cerium Oxide Nanoparticles for Colorimetric Detection of Nucleic Acids.

ABSTRACT: In recent years, cerium oxide (CeO2) nanoparticles (NPs) have drawn significant attention owing to their intrinsic enzyme mimetic properties, which make them powerful tools for biomolecular detection. In this work, we evaluated the effect of pyrophosphate (PPi) on the oxidase activity of CeO2 NPs. The presence of PPi was found to enhance the oxidase activity of CeO2 NPs, with enhanced colorimetric signals. This particular effect was then used for the colorimetric detection of target nucleic acids. Overall, the PPi-enhanced colorimetric signals of CeO2 NPs oxidase activity were suppressed by the presence of the target nucleic acids. Compared with previous studies using CeO2 NPs only, our proposed system significantly improved the signal change (ca. 200%), leading to more sensitive and repro

SUBMITTER: Kim S 

PROVIDER: S-EPMC8623087 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature