Expression and Functionality of Connexin-Based Channels in Human Liver Cancer Cell Lines.

ABSTRACT: Liver cancer cell lines are frequently used in vitro tools to test candidate anti-cancer agents as well as to elucidate mechanisms of liver carcinogenesis. Among such mechanisms is cellular communication mediated by connexin-based gap junctions. The present study investigated changes in connexin expression and gap junction functionality in liver cancer in vitro. For this purpose, seven human liver cancer cell lines, as well as primary human hepatocytes, were subjected to connexin and gap junction analysis at the transcriptional, translational and activity level. Real-time quantitative reverse transcription polymerase chain reaction analysis showed enhanced expression of connexin43 in the majority of liver cancer cell lines at the expense of connexin32 and connexin26. Some of these changes

SUBMITTER: Leroy K 

PROVIDER: S-EPMC8623148 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature