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Lycium Barbarum Polysaccharide-Iron (III) Chelate as Peroxidase Mimics for Total Antioxidant Capacity Assay of Fruit and Vegetable Food.


ABSTRACT: Quantitative evaluation of the antioxidant capacity of foods is of great significance for estimating food's nutritional value and preventing oxidative changes in food. Herein, we demonstrated an easy and selective colorimetric method for the total antioxidant capacity (TAC) assay based on 3,3',5,5'-tetramethyl-benzidine (TMB), hydrogen peroxide (H2O2) and synthetic Lycium barbarum polysaccharide-iron (III) chelate (LBPIC) with high peroxidase (POD)-like activity. The results of steady-state kinetics study showed that the Km values of LBPIC toward H2O2 and TMB were 5.54 mM and 0.16 mM, respectively. The detection parameters were optimized, and the linear interval and limit of detection (LOD) were determined to be 2-100 μM and 1.51 μM, respectively. Additionally, a subsequent study of the determination of TAC in six commercial fruit and vegetable beverages using the established method was successfully carried out. The results implied an expanded application of polysaccharide-iron (III) chelates with enzymatic activity in food antioxidant analysis and other biosensing fields.

SUBMITTER: Shi S 

PROVIDER: S-EPMC8623380 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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<i>Lycium Barbarum</i> Polysaccharide-Iron (III) Chelate as Peroxidase Mimics for Total Antioxidant Capacity Assay of Fruit and Vegetable Food.

Shi Shuo S   Feng Jianxing J   Liang Yanmin Y   Sun Hao H   Yang Xuewei X   Su Zehui Z   Luo Linpin L   Wang Jianlong J   Zhang Wentao W  

Foods (Basel, Switzerland) 20211114 11


Quantitative evaluation of the antioxidant capacity of foods is of great significance for estimating food's nutritional value and preventing oxidative changes in food. Herein, we demonstrated an easy and selective colorimetric method for the total antioxidant capacity (TAC) assay based on 3,3',5,5'-tetramethyl-benzidine (TMB), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and synthetic <i>Lycium barbarum</i> polysaccharide-iron (III) chelate (LBPIC) with high peroxidase (POD)-like activity. The  ...[more]

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