Highly Stable Potentiometric (Bio)Sensor for Urea and Urease Activity Determination.

ABSTRACT: There is growing interest for bioanalytical tools that might be designed for a specific user, primarily for research purposes. In this perspective, a new, highly stable potentiometric sensor based on glassy carbon/polyazulene/NH4+-selective membrane was developed and utilized for urease activity determination. Urease-urea interaction studies were carried out and the Michaelis-Menten constant was established for this enzymatic reaction. Biofunctionalization of the ammonium ion-selective sensor with urease lead to urea biosensor with remarkably good potential stability (drift coefficient ~0.9 mV/h) and short response time (t95% = 36 s). The prepared biosensor showed the Nernstian response (S = 52.4 ± 0.7 mV/dec) in the urea concentration range from 0.01 to 20 mM, stable for the experimental

SUBMITTER: Urbanowicz M 

PROVIDER: S-EPMC8623495 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature