Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against Coxiella burnetii.

ABSTRACT: The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine C. burnetii proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect C. burneti

SUBMITTER: Jeske R 

PROVIDER: S-EPMC8623512 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature