Quantitative Phosphoproteomics of cipk3/9/23/26 Mutant and Wild Type in Arabidopsis thaliana.

ABSTRACT: CBL-interacting protein kinases 3/9/23/26 (CIPK3/9/23/26) are central regulation components of magnesium ion homeostasis. CBL2/3 interacts with CIPK3/9/23/26, which phosphorylates their downstream targets, suggesting that protein phosphorylation is a key factor influencing the maintenance of cellular magnesium homeostasis in higher plants. The cipk3/9/23/26 quadruple mutant is very sensitive to high levels of magnesium. In this study, TMT quantitative phosphoproteomics were used to compare the global variations in phosphoproteins in wild type and cipk3/9/23/26 quadruple mutant seedlings of Arabidopsis thaliana, and 12,506 phosphorylation modification sites on 4537 proteins were identified, of which 773 phosphorylated proteins e

SUBMITTER: Yin Z 

PROVIDER: S-EPMC8623713 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature