Precision DNA Mixture Interpretation with Single-Cell Profiling.

ABSTRACT: Wet-lab based studies have exploited emerging single-cell technologies to address the challenges of interpreting forensic mixture evidence. However, little effort has been dedicated to developing a systematic approach to interpreting the single-cell profiles derived from the mixtures. This study is the first attempt to develop a comprehensive interpretation workflow in which single-cell profiles from mixtures are interpreted individually and holistically. In this approach, the genotypes from each cell are assessed, the number of contributors (NOC) of the single-cell profiles is estimated, followed by developing a consensus profile of each contributor, and finally the consensus profile(s) can be used for a DNA database search or comparing with known profiles to determine their potential sou

SUBMITTER: Ge J 

PROVIDER: S-EPMC8623868 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature