Transcriptome-Wide Profile of 25-Hydroxyvitamin D₃ in Primary Immune Cells from Human Peripheral Blood.

ABSTRACT: Vitamin D₃ is an essential micronutrient mediating pleiotropic effects in multiple tissues and cell types via its metabolite 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃), which activates the transcription factor vitamin D receptor. In this study, we used peripheral blood mononuclear cells (PBMCs) obtained from five healthy adults and investigated transcriptome-wide, whether the precursor of 1,25(OH)₂D₃, 25-hydroxyvitamin D₃ (25(OH)D₃), has gene regulatory potential on its own. Applying thresholds of >2 in fold change of gene expression and <0.05 as a false discovery rate, in this ex vivo approach the maximal physiological concentration of 25(OH)D₃ (250 nM (nmol/L)) none of the study participants h

SUBMITTER: Hanel A 

PROVIDER: S-EPMC8624141 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature