A Fluorescence Kinetic-Based Aptasensor Employing Stilbene Isomerization for Detection of Thrombin.

ABSTRACT: It is important to detect thrombin due to its physiological and pathological roles, where rapid and simple analytical approaches are needed. In this study, an aptasensor based on fluorescence attenuation kinetics for the detection of thrombin is presented, which incorporates the features of stilbene and aptamer. We designed and synthesized an aptasensor by one-step coupling of stilbene compound and aptamer, which employed the adaptive binding of the aptamer with thrombin to cause a change in stilbene fluorescence attenuation kinetics. The sensor realized detection of thrombin by monitoring the variation in apparent fluorescence attenuation rate constant (k_app), which could be further used for probing of enzyme-aptamer binding. In comprehensive studies, the developed aptas

SUBMITTER: Zeng X 

PROVIDER: S-EPMC8624160 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature