L-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance.

ABSTRACT: Lactic acid is mainly used to produce bio-based, bio-degradable polylactic acid. For industrial production of lactic acid, engineered Saccharomyces cerevisiae can be used. To avoid cellular toxicity caused by lactic acid accumulation, pH-neutralizing agents are used, leading to increased production costs. In this study, lactic acid-producing S. cerevisiae BK01 was developed with improved lactic acid tolerance through adaptive laboratory evolution (ALE) on 8% lactic acid. The genetic basis of BK01 could not be determined, suggesting complex mechanisms associated with lactic acid tolerance. However, BK01 had distinctive metabolomic traits clearly separated from the parental strain, and lactic acid production was improved by 17% (from 102 g/L to 119 g/L). To the best of our know

SUBMITTER: Jang BK 

PROVIDER: S-EPMC8624227 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature