Mass Spectrometry-Based System for Identifying and Typing Norovirus Major Capsid Protein VP1.

ABSTRACT: Norovirus-associated diseases are the most common foodborne illnesses worldwide. Polymerase chain reaction-based methods are the primary diagnostics for clinical samples; however, the high mutation rate of norovirus makes viral amplification and genotyping challenging. Technological advances in mass spectrometry (MS) make it a promising tool for identifying disease markers. Besides, the superior sensitivity of MS and proteomic approaches may enable the detection of all variants. Thus, this study aimed to establish an MS-based system for identifying and typing norovirus. We constructed three plasmids containing the major capsid protein VP1 of the norovirus GII.4 2006b, 2006a, and 2009a strains to produce virus-like particles for use as standards. Digested peptide signals were collected usin

SUBMITTER: Chu PY 

PROVIDER: S-EPMC8624548 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature