Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients.

ABSTRACT: Turner syndrome (TS) is a genetic disorder in females with X Chromosome monosomy associated with highly variable clinical features, including premature primary gonadal failure leading to ovarian dysfunction and infertility. The mechanism of development of primordial germ cells (PGCs) and their connection with ovarian failure in TS is poorly understood. An in vitro model of PGCs from TS would be beneficial for investigating genetic and epigenetic factors that influence germ cell specification. Here we investigated the potential of reprogramming peripheral mononuclear blood cells from TS women (PBMCs-TS) into iPSCs following in vitro differentiation in hPGCLCs. All hiPSCs-TS lines demonstrated pluripotency state and were capable of differentiation into three embryonic layers (ectoderm, endod

SUBMITTER: de Souza AF 

PROVIDER: S-EPMC8624672 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature