Application of TaqMan Real-Time PCR for Detecting 'Candidatus Arsenophonus Phytopathogenicus' Infection in Sugar Beet.

ABSTRACT: The γ-proteobacterium 'Candidatus Arsenophonus phytopathogenicus' is assigned as the major pathogen of "Syndrome des basses richesses", a sugar beet disease characterised by a reduction in the sugar content of taproots and biomass yield. Despite the economic impact of this bacteriosis, diagnostics for this important pathogen currently rely on end-point PCR detection. Herein, we introduce a TaqMan qPCR for diagnostics of the agent targeting genes encoding a heat shock protein of the Hsp20 family and mannose-6-phosphate isomerase. Quantitation with synthetic oligonucleotides as standard showed that the developed TaqMan qPCR assays enable the detection of up to 100 target copies. A comparison between the TaqMan qPCR and end-point PCR for 'Ca. A. phytopathogenicus' detection was

SUBMITTER: Zubert C 

PROVIDER: S-EPMC8625364 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature