Detection of pks Island mRNAs Using Toehold Sensors in Escherichia coli.

ABSTRACT: Synthetic biologists have applied biomolecular engineering approaches toward the goal of novel biological devices and have shown progress in diverse areas of medicine and biotechnology. Especially promising is the application of synthetic biological devices towards a novel class of molecular diagnostics. As an example, a de-novo-designed riboregulator called toehold switch, with its programmability and compatibility with field-deployable devices showed promising in vitro applications for viral RNA detection such as Zika and Corona viruses. However, the in vivo application of high-performance RNA sensors remains challenging due to the secondary structure of long mRNA species. Here, we introduced 'Helper RNAs' that can enhance the functionality of toehold switch sensors by mitigating the eff

SUBMITTER: Heo T 

PROVIDER: S-EPMC8625898 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature